Catalog Number - 68304-05P-3M

Cogent RP C4 Butyl™ Wide Pore is a reversed-phase HPLC stationary phase specifically designed for the separation of proteins, peptides, enzymes, and other large biomolecules. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, this 300 Å wide pore chemistry provides enhanced accessibility for larger molecules compared to standard 100 Å pore columns. The result is improved mass transfer, excellent recovery, and reliable chromatographic performance for biomolecular analyses.

68304-05P-3M is an HPLC column packed with 3 µm RP C4 Butyl™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 50 mm format. The short 50 mm length is optimized for rapid bioanalytical separations and high-throughput workflows, while the 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition and instrument configuration.

This column is ideal for proteins, peptides, enzymes, and other large hydrophobic or neutral compounds analyzed by reversed-phase HPLC. The wide pore structure allows biomolecules to fully access the bonded phase, making it an excellent choice for biopharmaceutical analysis, protein characterization, peptide mapping, and routine bioanalytical applications.

Key Benefits

• RP C4 Butyl™ Wide Pore stationary phase

• Fully double end capped

• Traditional butyl (C4) bonded phase

• Ultra-high purity silica support

• 300 Å wide pore design

• Optimized for proteins and peptides

• 3 µm particle technology

• Greater efficiency than 5 µm columns

• Fast analysis times

• Excellent for bioanalytical methods

Typical Applications

• Protein analysis

• Peptide analysis

• Enzyme characterization

• Bioanalytical methods

• Biopharmaceutical analysis

• Protein purity testing

• Peptide mapping

• Biomolecule separations

• Method development

• Reversed-phase HPLC

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4 Butyl™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 300 Å

• Column Dimensions: 4.6 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Designed for proteins, peptides, enzymes, and other large biomolecules

• 300 Å wide pores improve access to the bonded phase for larger molecules

• Suitable for conventional HPLC systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The short 50 mm length is ideal for rapid bioanalytical workflows

• Excellent choice for biopharmaceutical and biotechnology laboratories

• Particularly useful for large hydrophobic and neutral biomolecules

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